Figure 2
From: HNF1α inhibition triggers epithelial-mesenchymal transition in human liver cancer cell lines
Expression of EMT markers and transcription factors in HNF1α-inhibited HepG2 and Hep3B. A,D: mRNA expression levels were compared between HepG2 (A) or Hep3B (D) cells transfected with HNF1α siRNA (siH) and with control siRNA (siC) (two-tailed t-test) at 3 and 7 days after transfection. B,E: E-Cadherin, N-Cadherin and Vimentin expression was analyzed using western-blotting after 3 days of transfection with HNF1α siRNA (siH) or Control siRNA (siC) in HepG2 (B) or Hep3B (E). C: Immunofluorescence staining of E-Cadherin, and Fibronectin after 7 days of transfection of Control or HNF1α siRNA in HepG2. Scale bar 10 μm. F: Immunofluorescence staining of E-Cadherin and N-Cadherin after 3 days of transfection of Control or HNF1α siRNA in Hep3B. Scale bar 10 μm.