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Figure 5 | BMC Cancer

Figure 5

From: MiR-125b promotes proliferation and migration of type II endometrial carcinoma cells through targeting TP53INP1 tumor suppressor in vitro and in vivo

Figure 5

MiR-125b negatively regulated TP53INP1 protein levels in EC cells. A. Putative binding sites of miR-125b in the TP53INP1 3'UTR (white sequences) predicted by TargetScan. B. TP53INP1 protein levels were measured in EC cells at 72 h post-transfection by western blot assays. C. Potential binding site of miR-125b on TP53INP1 3'UTR in different species. The seed sequence is underlined. D. Sketch of the construction of pMIR-TP53INP1-3'UTR-WT or pMIR-TP53INP1-3'UTR-MT vectors. The mutant binding site is underlined and italicized. E. MiR-125bm down-regulated luciferase activities controlled by wild-type TP53INP1 3'UTR, but did not affect luciferase activity controlled by mutant TP53INP1 3'UTR (white sequences were mutated). The results are means of three independent experiments ± SD. (**P < 0.01 vs. blank; #P > 0.05 vs. blank).

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BMC Cancer

ISSN: 1471-2407

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