Figure 4

MiR-125b enhanced the mobility ability of EC cells using transwell migration assay. A. ishkawa cells were seeded in transwell filter. Migrated cells on the lower surface of the transwell filter were stained and counted after 24 h. Photographs were taken at 24 h post-migration (magnification, 100×). B. The numbers of migrated cells of ishikawa. Bars show mean ± SD. All experiments were repeated three times. *P < 0.01 vs. miR-125bm NC, untransfected. C. AN3CA cells seeded in transwell filter. Migrated cells on the lower surface of the transwell filter were stained and counted after 24 h. photographs were taken at 24 h post-migration (magnification, 100×). D. The numbers of migrated cells of AN3CA. Bars show mean ± SD. All experiments were repeated three times. *P < 0.01 vs. miR-125bi NC, untransfected.