Table 2 Intra-assay and inter-assay precision of the multiplex RT-qPCR for CK-19, MAGE A3, HER2 and PBGD.
From: Gene expression profile of circulating tumor cells in breast cancer by RT-qPCR
SKBR-3, Number of cells | Quantification cycle (Cq), Mean Cq (SD) | Copies, Mean Copies (SD) | CV% |
|---|---|---|---|
CK-19 | Intra assay precision (n = 3) | ||
1 | 29.75 (0.15) | 218 (± 22) | 10 |
10 | 25.90 (0.17) | 3.12 (± 0.34) × 103 | 11 |
100 | 21.87 (0.17) | 5.07 (± 0.61) × 104 | 12 |
1000 | 18.37 (0.48) | 5.8 (± 1.8) × 105 | 31 |
| Â | Inter assay precision (n = 5) | ||
100 | 21.70 (0.27) | 5.8 (± 1.2) × 104 | 21 |
MAGE A3 | Intra assay precision (n = 3) | ||
1 | 34.51 (0.58) | 43 (± 14) | 33 |
10 | 30.22 (0.31) | 6.0 (± 1.1) × 102 | 18 |
100 | 26.58 (0.22) | 5.79 (± 0.75) × 103 | 13 |
1000 | 23.25 (0.42) | 4.7 (± 1.1) × 104 | 23 |
| Â | Inter assay precision (n = 5) | ||
100 | 26.41 (0.21) | 5.18 (± 0.87) × 102 | 17 |
HER-2 | Intra assay precision (n = 3) | ||
1 | 33.38 (0.39) | 14 (± 4) | 29 |
10 | 28.81 (0.10) | 321 (± 20) | 6.2 |
100 | 24.72 (0.11) | 5.33 (± 0.38) × 103 | 7.2 |
1000 | 21.48 (0.37) | 5.0 (± 1.2) × 104 | 24 |
| Â | Inter assay precision (n = 5) | ||
100 | 24.43 (0.39) | 6.6 (± 1.6) × 103 | 24 |
PBGD | Intra assay precision (n = 3) | ||
10 | 32.43(0.16) | 27 (± 3) | 11 |
100 | 29.31(0.020) | 227 (± 3) | 1.3 |
1000 | 26.26 (0.41) | 19 (± 4.8) × 102 | 25 |
| Â | Inter assay precision (n = 5) | ||
100 | 29.28 (0.27) | 324 (± 60) | 19 |