Skip to main content

Table 2 Intra-assay and inter-assay precision of the multiplex RT-qPCR for CK-19, MAGE A3, HER2 and PBGD.

From: Gene expression profile of circulating tumor cells in breast cancer by RT-qPCR

SKBR-3,
Number of cells
Quantification cycle (Cq),
Mean Cq (SD)
Copies,
Mean Copies (SD)
CV%
CK-19 Intra assay precision (n = 3)
1 29.75 (0.15) 218 (± 22) 10
10 25.90 (0.17) 3.12 (± 0.34) × 103 11
100 21.87 (0.17) 5.07 (± 0.61) × 104 12
1000 18.37 (0.48) 5.8 (± 1.8) × 105 31
  Inter assay precision (n = 5)
100 21.70 (0.27) 5.8 (± 1.2) × 104 21
MAGE A3 Intra assay precision (n = 3)
1 34.51 (0.58) 43 (± 14) 33
10 30.22 (0.31) 6.0 (± 1.1) × 102 18
100 26.58 (0.22) 5.79 (± 0.75) × 103 13
1000 23.25 (0.42) 4.7 (± 1.1) × 104 23
  Inter assay precision (n = 5)
100 26.41 (0.21) 5.18 (± 0.87) × 102 17
HER-2 Intra assay precision (n = 3)
1 33.38 (0.39) 14 (± 4) 29
10 28.81 (0.10) 321 (± 20) 6.2
100 24.72 (0.11) 5.33 (± 0.38) × 103 7.2
1000 21.48 (0.37) 5.0 (± 1.2) × 104 24
  Inter assay precision (n = 5)
100 24.43 (0.39) 6.6 (± 1.6) × 103 24
PBGD Intra assay precision (n = 3)
10 32.43(0.16) 27 (± 3) 11
100 29.31(0.020) 227 (± 3) 1.3
1000 26.26 (0.41) 19 (± 4.8) × 102 25
  Inter assay precision (n = 5)
100 29.28 (0.27) 324 (± 60) 19