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Table 1 Oligonucleotide primers and probes

From: Gene expression profile of circulating tumor cells in breast cancer by RT-qPCR

Target gene Primer or probe Sequence (5'-3') Tm (°C) Amplicon
Size (bp)
Accession
No.
CK-19 Forward CGG GAC AAG ATT CTT GGT 52.4 142 Y00503
  Reverse CGT TGA TGT CGG CCT CCA 60.4   
  Probe TGT CCT GCA GAT CGA CAA CGC CC-FL 71.0   
   LCRed640-CTG GCT GC AGA TGA CTT CCG AAC C-PH 69.0   
MAGE A3 Forward TCG GTG AGG AGG CAA GGT T 60.1 147 NM_005362
  Reverse GAT GAC TCT GGT CAG GGC AA 57.8   
  Probe GGA GGA GCA CTG AAG GAG AAG ATC TGC-FL 69.2   
   LC610-AGT GGG TCT CCA TTG CCC AGC TC-PH 68.4   
HER-2 Forward AGC CGC GAG CAC CCA AGT 63.6 188 M11730
  Reverse CCT GCA CCT CCT GGA TAT CCT G 63.3   
  Probe CAC CTA CCT GCC CAC CAA TGC CA-FL 70.6   
   LC670-CTG TCC TTC CTG CAG GAT ATC CAG GA-PH 68.8   
PBGD Forward CCT GAG GCA CCT GGA AGG AG 62.5 163 NM_000190
  Reverse ATC TTC ATG CTG GGC AGG GA 61.8   
  Probe TGT GCC AGT AGC CGT GCA TAC AGC-FL 69.1   
   LC705-GAA GGA TGG GCA ACT GTA CCT GAC TG-PH 67.3   
hTERT Forward TCA AGG TGG ATG TGA CGG G 59.2 347 AF015950
  Reverse GGA CTT GCC CCT GAT GCG 61.7   
  Probe 6FAM - CGT GTT CTG GGG TTT GAT GAT GCT GGC GA - TMR 74,5   
hMAM Forward ACG GAT GAA ACT CTG AGC AAT G 59.4 106 U33147
  Reverse CAG TTC TGT GAG CCA AAG GT 54.9   
  Probe 6FAM-TGA GGT GTT TAT GCA ATT AAT ATA TGA CAG CAG TC -XT- -PH 64.2   
TWIST1 Forward GGC CGG AGA CCT AGA TGT 54,2 150 NM_000474
  Reverse CAC GCC CTG TTT CTT TGA AT 57,8   
  Probe 6FAM-TGG ACA GTC TAG AGA CT
C TGG AGC TGG-BBQ
66,6