Table 1 Oligonucleotide primers and probes
From: Gene expression profile of circulating tumor cells in breast cancer by RT-qPCR
Target gene | Primer or probe | Sequence (5'-3') | Tm (°C) | Amplicon Size (bp) | Accession No. |
|---|---|---|---|---|---|
CK-19 | Forward | CGG GAC AAG ATT CTT GGT | 52.4 | 142 | Y00503 |
| Â | Reverse | CGT TGA TGT CGG CCT CCA | 60.4 | Â | Â |
| Â | Probe | TGT CCT GCA GAT CGA CAA CGC CC-FL | 71.0 | Â | Â |
| Â | Â | LCRed640-CTG GCT GC AGA TGA CTT CCG AAC C-PH | 69.0 | Â | Â |
MAGE A3 | Forward | TCG GTG AGG AGG CAA GGT T | 60.1 | 147 | NM_005362 |
| Â | Reverse | GAT GAC TCT GGT CAG GGC AA | 57.8 | Â | Â |
| Â | Probe | GGA GGA GCA CTG AAG GAG AAG ATC TGC-FL | 69.2 | Â | Â |
| Â | Â | LC610-AGT GGG TCT CCA TTG CCC AGC TC-PH | 68.4 | Â | Â |
HER-2 | Forward | AGC CGC GAG CAC CCA AGT | 63.6 | 188 | M11730 |
| Â | Reverse | CCT GCA CCT CCT GGA TAT CCT G | 63.3 | Â | Â |
| Â | Probe | CAC CTA CCT GCC CAC CAA TGC CA-FL | 70.6 | Â | Â |
| Â | Â | LC670-CTG TCC TTC CTG CAG GAT ATC CAG GA-PH | 68.8 | Â | Â |
PBGD | Forward | CCT GAG GCA CCT GGA AGG AG | 62.5 | 163 | NM_000190 |
| Â | Reverse | ATC TTC ATG CTG GGC AGG GA | 61.8 | Â | Â |
| Â | Probe | TGT GCC AGT AGC CGT GCA TAC AGC-FL | 69.1 | Â | Â |
| Â | Â | LC705-GAA GGA TGG GCA ACT GTA CCT GAC TG-PH | 67.3 | Â | Â |
hTERT | Forward | TCA AGG TGG ATG TGA CGG G | 59.2 | 347 | AF015950 |
| Â | Reverse | GGA CTT GCC CCT GAT GCG | 61.7 | Â | Â |
| Â | Probe | 6FAM - CGT GTT CTG GGG TTT GAT GAT GCT GGC GA - TMR | 74,5 | Â | Â |
hMAM | Forward | ACG GAT GAA ACT CTG AGC AAT G | 59.4 | 106 | U33147 |
| Â | Reverse | CAG TTC TGT GAG CCA AAG GT | 54.9 | Â | Â |
| Â | Probe | 6FAM-TGA GGT GTT TAT GCA ATT AAT ATA TGA CAG CAG TC -XT- -PH | 64.2 | Â | Â |
TWIST1 | Forward | GGC CGG AGA CCT AGA TGT | 54,2 | 150 | NM_000474 |
| Â | Reverse | CAC GCC CTG TTT CTT TGA AT | 57,8 | Â | Â |
| Â | Probe | 6FAM-TGG ACA GTC TAG AGA CT C TGG AGC TGG-BBQ | 66,6 | Â | Â |