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Table 1 Comparison of the proportion of CD44+ cells as determined by flow cytometry to the relative mRNA expression determined by q-RT-PCR.

From: CD44 isoforms are heterogeneously expressed in breast cancer and correlate with tumor subtypes and cancer stem cell markers

Cell line

CD44+ by FCM (% positive cells)1

Relative mRNA expression of total CD44

MCF7

15.1

1.00

MDA-MB-361

79.6

0.95

BT-474

0

0.09

ZR-75-1

6.00

0.39

SK-BR-3

7.42

0.04

HCC1428

65.8

8.96

MDA-MB-436

97.3

9.37

L56Br-C1

49.5

2.04

MDA-MB-231

99.2

9.35

HCC1937

96.0

9.18

PMC42

98.4

3.89

JIMT-1

99.8

17.4

MCF10A

99.7

6.21

  1. The relative mRNA expression was calculated using the cell line MCF7 as reference.
  2. 1 Appropriate isotype control was used to set the threshold for CD44 positive cells.