Figure 7

The phenanthridine PJ-34 did not affect the cell-cycle of human normal proliferating cells. Flow cytometry analysis shows that PJ-34 did not affect the cell cycle of human mammary epithelial cells MCF-10, adipose-derived mesenchymal stroma cells prepared from thymus, and human umbilical vein endothelial cells (Huvec). Cells were incubated for the indicated periods with the indicated concentrations of PJ-34 applied 24 hours after seeding. For each cell type, similar results were obtained in experiments repeated 3 times (Methods).