Figure 5

Flow cytometry indicating G2/M arrest and cell death in the multi-centrosomal human cancer cells. PJ-34 was applied 24 hours after seeding to human colon cancer DLD-1 cells, lung cancer H1299 cells and ovarian cancer HeyA8 cells at the indicated concentrations. Treated and control cells were analyzed 48, 72 and 96 hours after PJ-34 application (48 and 72 hours are presented only for DLD1). The effect of PJ-34 on cell eradication (sub-G1 population) and the kinetics of S-phase entry and G2/M transition were assayed by the percentage of cells at each of these phases. For each cell type, similar results were obtained in experiments repeated 3 times (Methods).