Figure 3

The phenanthrene derivatives Phen and Tiq-A interfered with supernumerary centrosomes clustering in MDA-231 cells. Confocal images present scattered centrosomes and aberrant arrangement of chromosomes in randomly selected fixed MDA-231 cells in mitosis. Top: These cells were permeabilized and immunolabeled for α- and γ-tubulin (labeling spindles (green) and centrosomes (red), respectively) 24 hours after application of Tiq-A or Phen at the indicated concentrations. Bottom: Bi-polar clustering of centrosomes in randomly selected fixed MDA-231 cells that were incubated for 48 hours with the non-phenanthrene derivatives ABT-888 and BSI-201, at the indicated concentrations, permeabilized and immunolabeled for α- and γ-tubulin. Chromosomes were labeled with DAPI reagent (blue).