Figure 6

Effects of the MK-0457 on TT cell colony formation in soft agar. (A) TT cells were plated in soft agar onto 3.5 cm Petri dishes in the absence or in the presence of MK-0457 (200 nM). Treated and non-treated plates were photographed after three weeks of incubation. The colony size was determined using the MetaVue software and those larger than 50 μm in diameter were scored. Photographs reported in the figure are representative of one out of three similar experiments each performed in triplicate. Scale bar, 100 μm. (B) Effects of MK-0457 on the number and size of TT colonies in soft agar. Data reported represent the mean ± SEM of three independent experiments. *p < 0.001