Effects of the MK-0457 on TT cell ploidy. Cells were incubated for 6 days with 200 nM MK-0457 or the vehicle (DMSO). At the end of the incubation time cells were fixed and analyzed by FACS. See also table 2. For the immunofluorescence experiments (insert) TT cells were exposed or not for 6 days to 200 nM MK-0457, then fixed and stained with DAPI and β-tubulin. Scale bar, 20 μm.