ELISpot analysis of FSP-recognition of T cells stimulated with Fc1. T cells (1 × 104 cells/well) were stimulated with 2 × 104 peptide loaded autologous CD40 Bs. Peptides were added at a final concentration of 10 μg/ml. Analysis was performed in sixplicates. The mean number of IFN-γ releasing T cells is given together with the standard deviation. Significant reactions in comparison to the negative control are indicated by *.