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Figure 4 | BMC Cancer

Figure 4

From: Clinical pharmacogenomic testing of KRAS, BRAF and EGFRmutations by high resolution melting analysis and ultra-deep pyrosequencing

Figure 4

Variants detected by ultra-deep pyrosequencing of KRAS amplicons. The variants frequency table (top) summarises the frequencies of the previously defined variants detected within each sample, with the corresponding number of combined reads shown between parentheses. Below, the plot of sequence variations detected in sample 97K and a partial image capture of the global alignment, displayed as consensus reads, are shown as an example of 454 sequencing output. The only change detected within this sample is the known c.34G > T mutation (G12C).

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