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Figure 1 | BMC Cancer

Figure 1

From: Clinical pharmacogenomic testing of KRAS, BRAF and EGFRmutations by high resolution melting analysis and ultra-deep pyrosequencing

Figure 1

Fusion primer design for KRAS screening with the GS Junior 454 system. The primers consist of three parts: the GS Junior 454 adaptor, containing the sequencing primers A and B and the sequencing key "TCAG" (underlined), followed by a 10-bp multiplex identifier (MID) tag that varies between samples, and the KRAS-specific sequence. Five primer pairs were designed using MIDs 1 to 5 and generating an amplicon of 409 bp that contains KRAS exon 2. Complete sequences of forward and reverse primers are shown for MID1.

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BMC Cancer

ISSN: 1471-2407

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