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Figure 5 | BMC Cancer

Figure 5

From: Snail1 induces epithelial-to-mesenchymal transition and tumor initiating stem cell characteristics

Figure 5

Snail1 regulates EMT and CSC characteristics in mesenchymal cells. (A) Epithelial cells were treated with Snail1 siRNA for 48 hours and mRNA expression was analyzed for E-cadherin, Zeb1, Zeb2, Bmi-1, Nanog, and CD44 normalized to Gapdh. Bars represent mean ± SEM of triplicates, *p < 0.01. Western blot analysis of Snail1, CD44, Nanog and β-actin, with data representative of two independent experiments. (B) Tumor-sphere formation assay of mesenchymal cells transfected with scrambled or Snail1 siRNA. Cells were cultured in low adherent plates (4 × magnification). (C) Wound assay of mesenchymal cells transfected with either scrambled or Snail1 siRNA. The number of cells migrated towards the wound was calculated. Data presented are mean ± SEM of triplicates, *p < 0.01.

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