Figure 2
From: Snail1 induces epithelial-to-mesenchymal transition and tumor initiating stem cell characteristics
Mesenchymal cells demonstrate up-regulation of TISC characteristics. (A) Microarray heatmap of TISC markers. (B) Relative expression of mRNAs encoding TISC genes Bmi-1, Nanog, Oct4, Abcg2, CD44, Cxcr4, and EpCam normalized to endogenous control Gapdh. Data represent mean ± SEM of triplicates, *p < 0.01. (C) Tumor-sphere assay was performed for two weeks using non-adherent plates. Phase contrast images are representation of three independent experiments (4 × magnification).