Figure 3

RT-qPCR analysis of lung cancer cell lines. Total RNA was isolated from several different lung cancer cells lines by the Trizol method (Invitrogen) according to the manufacturer's instructions, and qRT-PCR was performed using TaqMan MicroRNA Assay Kits (Applied Biosystems). The average expression levels of miR-96, miR-182, and miR-183 in tissues and sera were normalized using the 2-^ΔΔCt method relative to the average of U6 snRNA and U48 snRNA. The average Ct value of triplicates in three candidate miRNAs was calculated. *Indicates a significant difference compared to HBE cells (P < 0.05).