pEGFR activation by irradiation. The Western blot results were presented in figure form. Protein isolation was done 12 hrs after irradiation with 8 Gy. EGFR was stimulated by EGF and inhibited by AG1478 (AG) and the downstream pathways of EGFR: PI3K by LY294002 (LY), mTOR by rapamycin (Rapa) and MEK1 by PD98059 (PD), control. We find an up-regulation of phospho-EGFR after radiation.