Figure 3
Radiation induced migration. The number of migrating cells after irradiation with 0, 2, 5, and 8 Gy were determined by the wound healing assay for the HNSCC cell lines BHY, CAL-27 and HN. Cells were grown to confluent monolayers, wounded by scratching the surface as uniformly as possible with a pipette tip and afterwards irradiated. The number of migrated tumor cells in the wound was determined. The means and standard errors of 9 tests per time point and dose were calculated after 12 hrs. An increased time dependent migration of tumor cells after irradiation was observed after treatment compared to the control group of not irradiated cells (p < 0.009).