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Figure 5 | BMC Cancer

Figure 5

From: Purine Nucleoside Phosphorylase mediated molecular chemotherapy and conventional chemotherapy: A tangible union against chemoresistant cancer

Figure 5

Evaluation of treatment related effects on key proteins involved in cellular apoptotic pathways in OVCAR-3 cells: Cell lysates (50 μg) from treated and untreated cells were analysed by SDS-page/western blotting using the appropriate antibodies (panel A). The protein bands were quantified by densitometry relative to β-actin (loading control, representative image in the lowest panel) and expressed as the ratio of specific protein to beta-actin for different treatments (mean ± SEM, n = 2 for two separate experiments) (panel B). Note: only the representative beta-actin image is shown in panel A. For each protein tested, beta actin controls were included separately in the gel (not shown in panel A) and the ratios calculated individually (Panel B).

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