Panel A. Evaluation of Ad-transduction in different OC cell lines: Cells infected with AdGFP (10-500 pfu/cell) for 48 h were analysed for GFP expression by flow cytometry. Values represent mean (± SEM) of three experiments. Panel B:Evaluation of bystander effects associated with PNP-GDEPT in OC cells: Cells infected with AdPNP or AdGFP (control) at different moi followed by the prodrug treatment (Fludara @1 μg/mL(2.7 μM) were evaluated for cell viability (WST-1 assay) on day 5. Graphs show changes in cell viability relative to control (percentage of sham infected control cells) for different cell lines. Values represent mean (± SEM) of three experiments. Corresponding IC50 values of PNP-GDEPT in different ovarian cancer cell lines are also shown.