Figure 8

Histology of bones and lungs, and osteoclast staining of bones of the arthritic versus non-arthritic PyV MT mice. A-F: H&E staining of sections from the joints. A: C57BL/6 (no inflammation); B: C57BL/6 mice + CII at 9 wks (moderate inflammation); C: C57BL/6 mice + CII at 18 wks (moderate inflammation); D: control PyV MT mice (no inflammation); E: PyV MT mice + CII at 9 wks (erosion of articular cartilage and bone with severe inflammation); F: PyV MT mice + CII at 18 wks (severe inflammation in the phalangeal joints); G-L: Masson trichome staining of bone sections for presence of osteoclasts. G: C57/BL6 mice (no osteoclasts); H: C57BL/6 + CII at 9 wks (few osteoclasts marked with arrows); I: C57BL/6 mice + CII at 18 wks (few osteoclasts marked with arrows); J: PyV MT mice (no osteoclasts); K: PyV MT mice + CII at 9 wks (multiple osteoclasts marked with arrows); L: PyV MT mice + CII at 18 wks (multiple osteoclasts marked with arrows). M-R: H&E staining of sections from the lungs. M: control C57BL/6 (no inflammation); N: C57BL/6+CII at 9 wks(moderate inflammation); O: C57BL/6+CII at 18 wks(moderate inflammation); P: control PyV MT mice (no inflammation); Q: PyV MT mice + CII at 9 wks (severe inflammation); R: PyV MT mice + CII at 18 wks (severe inflammation). The H&E images of the joints and lungs were taken at 200× and 400× magnification respectively and the Masson Trichome bone images taken at 400× magnification. Representative of n = 10 mice is shown.