Figure 1

Synergistic effect of paclitaxel and ABT-737 cultured cells. Fibroblasts (black square), HepT1 (black triangle) and HuH6 (black rhomb) cells were incubated with paclitaxel at seven different concentrations alone (A) and in combination with 0.3 μM ABT-737 (B). Relative cell viability was determined 72 hrs later in a MTT assay. No toxicity was observed in fibroblasts. For HB cells synergistic effects of combined treatment could be observed. Apoptosis was detected by Caspase 3 activity 24 h after combination treatment of HuH6 and HepT1 cells (C). Enhanced Caspase 3 activity was found in HuH6 cells incubated with paclitaxel and 0.3 μM ABT-737 when compared with paclitaxel alone.