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Table 1 Cytotoxicity of peptides to various cell lines and TfR expression

From: A novel transferrin receptor-targeted hybrid peptide disintegrates cancer cell membrane to induce rapid killing of cancer cells

Cell line   IC50
Lytic peptide alone
(μM)**
TfR-lytic peptide
IC50Ratio
Lyttic/TfR-lytic
MFI*
mean
Statistical
Difference
vs HC
   mean mean    
Cancer cells Organ      
BT474 Breast 34.5 ± 3.8 8.8 ± 3.3 3.9 99.9 ± 24.9 < 0.05
T47D Breast 14.1 ± 3.6 4.0 ± 0.2 3.6 101.4 ± 32.4 < 0.05
SK-BR-3 Breast 25.7 ± 0.6 6.5 ± 0.9 4.0 79.7 ± 21.2 < 0.05
MDA-MB-231 Breast 27.0 ± 1.5 7.8 ± 0.8 3.4 98.1 ± 23.9 < 0.05
BT20 Breast 20.0 ± 2.9 4.8 ± 0.4 4.2 91.5 ± 14.6 < 0.05
ZR75-1 Breast 19.4 ± 1.7 9.3 ± 2.6 2.1 46.4 ± 9.0 0.08
SF295 Brain 19.1 ± 2.9 6.3 ± 1.8 3.0 56.9 ± 17.6 0.08
SN19 Brain 21.9 ± 4.0 7.8 ± 0.5 2.8 75.3 ± 10.0 < 0.05
U251 Brain 17.0 ± 1.4 7.0 ± 2.0 2.4 56.3 ± 22.8 0.13
HuCCT-1 Bile duct 17.1 ± 2.8 8.3 ± 2.5 2.0 46.2 ± 4.6 < 0.05
LNCap Prostate 15.8 ± 1.5 6.2 ± 0.0 2.5 57.8 ± 5.9 < 0.05
COLO587 Pancreas 13.5 ± 1.2 6.4 ± 1.3 2.1 46.4 ± 15.8 0.18
Normal cells       
Hepatocyte (HC) Liver 59.2 ± 5.0 54.7 ± 3.8 1.1 35.5 ± 4.2  
Pancreatic epithelical (PE) cell Pancreas 59.6 ± 7.2 51.2 ± 3.6 1.2 2.8 ± 0.1  
  1. *MFI, mean fluorescence intensity; this is the extent of binding of the PE-conjugated anti-TfR monoclonal antibody to cells. The value of MFI is represented by means ± SD from independent 3 time assays.
  2. **The value of IC50 is represented by means ± SD from triplicate determinations, and the assay was repeated three times.