Figure 5

Characterization of the cancer cell death mechanism by TfR-lytic hybrid peptide: (A) Cancer T47D and normal PE cells were incubated with TfR-lytic peptide (10 μM) or lytic peptide (10 μM) for 3 h, then analyzed by dual-color flow cytometry for annexin V labeling and propidium iodide (PI) staining. (B) T47D and PE cells were incubated with TfR-lytic peptide (10 μM) or lytic peptide (10 μM) for 3 h, then analyzed by dual-color flow cytometry for caspase 3&7 and PI staining. Cell population values (%) are shown caspase 3&7-positive. The results are represented as means ± SD (bars) from triplicate determinations. (C) T47D cells labeled with the mitochondrial-transmembrane-potential-sensitive fluorescent dye JC-1 were treated with TfR-lytic peptide (upper panel) or lytic peptide (middle panel), or left untreated (lower panel), for 2 h, and analyzed for transmembrane potential by flow cytometry.