Figure 5

Microarray and protein expression analysis of endothelial cells treated with VEGF ₁₂₁ /rGel. A, Validation of the microarray analysis by PCR is shown. Upregulation of genes for E-selectin, TNFAIP3, NF-κBIα and SCYA2 were validated by RT-PCR. GAPDH levels were assessed as a control. B, RNA from PAE/VEGFR-2 cells untreated or treated with VEGF₁₂₁/rGel for the periods indicated were examined by PCR for upregulation of E-selectin. GAPDH primers were used as a control for loading. RNA levels of E-selectin were all upregulated in PAE/VEGFR-2 cells, as well as in HUVECs. C, Protein levels of E-selectin are only slightly upregulated compared to E-selectin RNA.