Downregulation of TFPI in breast cancer cells induces tyrosine phosphorylation signaling and increases metastatic growth by stimulating cell motility

Table 1 Stable downregulation of both isoforms of TFPI in Sum102 and MDA-MB-231 breast cancer cells.

		TFPIα/β mRNA downregulation, % of control	Total TFPI ag	Total TFPI ag, PI-PLC/ Untreated	Free TFPI ag, PI-PLC/ Untreated
Sum102 [15]	pSiRPG-shRNA4	60/51	13.0
	pSiRPG-shRNA6	35/36	28.1	1.3/n.d	n.d/n.d
	pSiRPG-shRNA7	46/42	19.0	1.1/n.d	0.1/n.d
	pSiRPG (control)	0/0	56.7	2.4/n.d	0.1/0.1
MDA-MB-231	pSiRPG-shRNA4	62/58	3.6
	pSiRPG-shRNA6	26/36	12.0	0.7/0.2	0.1/0.2
	pSiRPG-shRNA7	41/46	8.2	0.4/0.2	0.1/0.1
	pSiRPG (control)	0/0	17.2	1.5/0.3	0.2/0.2

Sum102 and MDA-MB-231 breast cancer cells were transduced with vectors containing shRNAs against TFPI or empty vector pSiRPG as a control. Relative TFPIα and β mRNA levels were quantified using qRT-PCR and secreted protein (ag) with total TFPI ELISA kit. GPI attached TFPI was measured in the supernatant after PI-PLC treatment using total and free TFPI ELISA kits (n.d = not detected). Ag levels were corrected against cellular total protein and presented as ng TFPI/mg total protein.

ISSN: 1471-2407