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Table 1 Stable downregulation of both isoforms of TFPI in Sum102 and MDA-MB-231 breast cancer cells.

From: Downregulation of TFPI in breast cancer cells induces tyrosine phosphorylation signaling and increases metastatic growth by stimulating cell motility

   TFPIα/β mRNA
downregulation,
% of control
Total TFPI ag Total TFPI ag,
PI-PLC/
Untreated
Free TFPI ag,
PI-PLC/
Untreated
Sum102 [15] pSiRPG-shRNA4 60/51 13.0   
  pSiRPG-shRNA6 35/36 28.1 1.3/n.d n.d/n.d
  pSiRPG-shRNA7 46/42 19.0 1.1/n.d 0.1/n.d
  pSiRPG (control) 0/0 56.7 2.4/n.d 0.1/0.1
MDA-MB-231 pSiRPG-shRNA4 62/58 3.6   
  pSiRPG-shRNA6 26/36 12.0 0.7/0.2 0.1/0.2
  pSiRPG-shRNA7 41/46 8.2 0.4/0.2 0.1/0.1
  pSiRPG (control) 0/0 17.2 1.5/0.3 0.2/0.2
  1. Sum102 and MDA-MB-231 breast cancer cells were transduced with vectors containing shRNAs against TFPI or empty vector pSiRPG as a control. Relative TFPIα and β mRNA levels were quantified using qRT-PCR and secreted protein (ag) with total TFPI ELISA kit. GPI attached TFPI was measured in the supernatant after PI-PLC treatment using total and free TFPI ELISA kits (n.d = not detected). Ag levels were corrected against cellular total protein and presented as ng TFPI/mg total protein.