Figure 7
Phosphorylation status of signaling molecules in the total lysate of transduced cells after FBS stimulation. Cells were starved for 5.5 hours before 1-2 × 106 cells were stimulated with 10% FBS for indicated time points. General tyrosine phosphorylation in Sum102 (A) and MDA-MB-231 (B) cells expressing shRNA6 (lane 1), -7 (lane 2) or empty vector control (lane 3) and (C) in MDA-MB-231 cells expressing shRNA7β (lane 1), -9β (lane 2) or empty vector control (lane 3), detected using anti-4G10 antibody. Src family phosphorylation in MDA-MB-231 cells expressing (D) shRNA6 (lane 1), -7 (lane 2) or empty vector control (lane 3) and (E) shRNA7β (lane 1), -9β (lane 2) or empty vector control (lane 3), detected using anti-phospho-Src antibody. c-Src was used as loading control. One representative experiment of three is shown.