Invasion of transduced cells measured using modified Boyden chambers. Cells were seeded in matrigel pre-coated chambers and incubated for 48/24 hours (Sum102/MDA-MB-231) at 37°C before stained with Calcein AM. Results are presented as mean relative invasion + SEM of three independent experiments. (A) Sum102 cells (n ≥ 8, **p = .0063 and ***p ≤ .0001 against pSiRPG) and (B) MDA-MB-231 cells (n ≥ 8, ***p < .0001 against pSiRPG) with TFPI downregulated. (C) MDA-MB-231 cells with TFPIβ downregulated (n ≥ 8, *p = .0227 and **p = .0098 against pSiRPG).