Self-sustained growth of Sum102 cells after downregulation of TFPI. (A) Sum102 cells expressing shRNA4 or -7 against TFPI or pSiRPG empty control were seeded at 10000 cells/well in 12-wells trays and grown for a minimum of 8 days. The results are presented as mean cell count (n = 9) + SEM of three independent experiments (**p = .0039 and ***p = .0003 against pSiRPG). (B and C) For colony formation assays, transduced Sum102 cells were seeded individually in soft-agar and grown for 2 to 4 weeks. Colonies were counted using ImageJ and the results are presented as mean number of colonies (n = 3) + SD of one representative experiment (*p < .046 and ***p = .0006 against pSiRPG). Scale bar = 1 cm.