Identification of RASSF1A and its splicing variants by PCR. Electropherograms of PCR products obtained by amplification of cDNA. PCR products were loaded on microfluidic DNA 1000 chip and run on a 2100 Bioanalyzer instrument (Agilent Technologies). The amplicon of isoform A is 229 bp, both isoforms D and E generate a 241 bp peak, due to the insertion of 12 bp to the sequence of isoform A, and therefore cannot be separated, while the amplicon of isoform F is the shortest one (118 bp). Peaks at 15bp and 1500 bp are the lower and upper size markers, respectively. Case T9 expresses isoform A, N5 expresses isoform A and D/E, T7 expresses isoform A and F.