Figure 6From: Methylation-associated down-regulation of RASSF1A and up-regulation of RASSF1Cin pancreatic endocrine tumors Identification of RASSF1A and its splicing variants by PCR. Electropherograms of PCR products obtained by amplification of cDNA. PCR products were loaded on microfluidic DNA 1000 chip and run on a 2100 Bioanalyzer instrument (Agilent Technologies). The amplicon of isoform A is 229 bp, both isoforms D and E generate a 241 bp peak, due to the insertion of 12 bp to the sequence of isoform A, and therefore cannot be separated, while the amplicon of isoform F is the shortest one (118 bp). Peaks at 15bp and 1500 bp are the lower and upper size markers, respectively. Case T9 expresses isoform A, N5 expresses isoform A and D/E, T7 expresses isoform A and F.Back to article page