Expression of RASSF1A and RASSF1C in PET, normal pancreas and PET cell lines. The expression levels of RASSF1A (A) and of RASSF1C (C) in 13 PET (black bars) and matched normal pancreas (white bars) are shown. Expression data are the mean of three measures obtained by quantitative RT-PCR. Data were normalized using the expression level of the gene RPLPO as an internal reference. Data were analyzed according to the comparative method and standard (= 1) was represented by the average expression of all samples. Numbers under the bars refer to PET cases listed in Table 1. Expression level of RASSF1A (B) and of RASSF1C (D) (mean ± SD) in PET cell lines QGP1, BON, CM are represented by white bars for control untreated (CTRL) and dark grey bars for 5'-aza-2'-deoxycytidine (DAC) treated cells. In (A) and (C) an asterisk indicates a significant difference of expression of RASSF1A and RASSF1C in normal with respect to PET (t-test, P < 0.05) (see Additional file 2, Table S2 for expression data and statistics). In (B) and (D) an asterisk indicates a significant difference of expression in treated cells with respect to the same cells untreated (t-test, P < 0.05).