The effects of antisense RRIG1 in breast cancer T47D cells. All experiments were repeated at least once with similar results. A, Semiquantitative RT-PCR. The gene-transfected cells were grown in G418-containing medium for 5 days, and RNA was then isolated from the cells and subjected to semiquantitative RT-PCR analysis of RRIG1, SH3GLB2, and GAPDH expression. B, MTT assay. The gene-transfected cells were grown in G418-containing medium for 1 or 5 days, and cell viability was measured using the MTT assay. C, Invasion assay. These gene-transfected breast cancer cells were grown in G418-containing medium and then subjected to the cell invasion assay in Boyden chambers with Matrigel for 48 h. The invaded cells were stained with 1% crystal violet solution and then counted and summarized. Vec, vector-only; AS, antisense RRIG1 cDNA; *p < 0.05 vs. the control.