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Figure 1 | BMC Cancer

Figure 1

From: Protein-bound polysaccharide from Phellinus linteus inhibits tumor growth, invasion, and angiogenesis and alters Wnt/β-catenin in SW480 human colon cancer cells

Figure 1

PL inhibited cell growth and Wnt/β-catenin signaling in SW480 human colon cancer cells. A, SW480 cells were treated with various concentrations of PL for the indicated times. At the indicated time points, cell growth was measured by the MTT assay as described in Methods. The data represent the mean of three independent experiments ± SD. ***, P < 0.001 versus control. B, Cells were treated with various concentrations of PL (125-500 μg/mL). After 24 h, protein samples were prepared and each sample was electrophoresed in an SDS-PAGE gel and immuno-blotted to detect β-catenin and cyclin D1. The blots shown are representative of three independent experiments with similar results. C, SW480 cells were treated with PL (250 μg/mL) and immuno-stained for β-catenin. Representative images were obtained by confocal microscopy. D, SW480 cells transiently transfected with p-luc TCF/LEF and treated with the indicated concentrations of PL. Luciferase reporter activity was measured in cell extracts after 16 h of PL treatment. Error bars, SD (n = 3). ***, P < 0.001 versus control.

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