TY - JOUR AU - Tricarico, Rossella AU - Crucianelli, Francesca AU - Alvau, Antonio AU - Orlando, Claudio AU - Sestini, Roberta AU - Tonelli, Francesco AU - Valanzano, Rosa AU - Genuardi, Maurizio PY - 2011 DA - 2011/07/21 TI - High resolution melting analysis for a rapid identification of heterozygous and homozygous sequence changes in the MUTYH gene JO - BMC Cancer SP - 305 VL - 11 IS - 1 AB - MUTYH-associated polyposis (MAP) is an autosomal recessive form of intestinal polyposis predisposing to colorectal carcinoma. High resolution melting analysis (HRMA) is a mutation scanning method that allows detection of heterozygous sequence changes with high sensitivity, whereas homozygosity for a nucleotide change may not lead to significant curve shape or melting temperature changes compared to homozygous wild-type samples. Therefore, HRMA has been mainly applied to the detection of mutations associated with autosomal dominant or X-linked disorders, while applications to autosomal recessive conditions are less common. SN - 1471-2407 UR - https://doi.org/10.1186/1471-2407-11-305 DO - 10.1186/1471-2407-11-305 ID - Tricarico2011 ER -