Figure 1

The TA area resembles matched primary tumors, human breast tumors and cancer cell lines. A. Hierarchical clustering of median centered gene expression from the TA area of the three cell lines (4T1, Cl66 and Cl66M2). B. The gene expression profile of primary syngeneic tumors derived using the 4T1, CI66 and 67NR mouse cell lines [23] resembled the TA area in our osteolytic breast cancer model as predicted by NTP algorithm (FDR < 0.2). Primary tumor samples and prediction significance (FDR < 0.2) are color coded and displayed at the top of the hierarchical cluster. C. The gene expression profile of primary human breast tumors [25] and D. human breast cancer cell lines resembled the TA area of either the 4T1 (up-regulated genes from 4T1 TA area, top bar in pink) or Cl66 (down-regulated genes from 4T1 TA area, top bar in green) of the osteolytic breast cancer model as predicted by NTP algorithm. Prediction significance (FDR < 0.2) for each sample is shown in the colored bar on the top of the hierarchical cluster. Samples predicted with FDR p > 0.2 do not resemble either the TA area or the TB interface.