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Table 5 Antibodies for Western Blot detection

From: Fatty acid binding proteins (FABPs) in prostate, bladder and kidney cancer cell lines and the use of IL-FABP as survival predictor in patients with renal cell carcinoma

Protein

Species

 

Supplier

Concentration

L-FABP

Rabbit IgG

polyclonal

Hycult biotechnology b.v., Uden, Netherlands

1 μg/ml

I-FABP

Rabbit IgG

polyclonal

Hycult biotechnology b.v., Uden, Netherlands

2 μg/ml

H-FABP

Mouse IgG1

monoclonal

Clone 66E2

Hycult biotechnology b.v., Uden, Netherlands

1 μg/ml

A-FABP

Rabbit IgG

polyclonal

Hycult biotechnology b.v., Uden, Netherlands

0.2 μg/ml

E-FABP

Rabbit IgG

polyclonal

Hycult biotechnology b.v., Uden, Netherlands

0.1 μg/ml

IL-FABP

Rabbit IgG

polyclonal

Hycult biotechnology b.v., Uden, Netherlands

1 μg/ml

B-FABP

Rabbit IgG

polyclonal

Hycult biotechnology b.v., Uden, Netherlands

1 μg/ml

β-actin

Mouse IgG2

monoclonal

Clone AC-74

Sigma-Aldrich Chemie GmbH, Munich, Germany

2.1 μg/ml

anti-rabbit IgG/HRP*

Goat

polyclonal

DakoCytomation, Glostrup, Denmark

0.12 μg/ml

anti-mouse IgG/HRP*

Rabbit

polyclonal

DakoCytomation, Glostrup, Denmark

0.53 μg/ml

  1. * HRP - Horseradish peroxidase