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Table 4 Sequences of primer sets and UPL probes

From: Fatty acid binding proteins (FABPs) in prostate, bladder and kidney cancer cell lines and the use of IL-FABP as survival predictor in patients with renal cell carcinoma

Gene

Accession number

Primer

Sequences (5'...3')

Product size[bp]

UPL probe*

L-FABP1

NM_001443

Forward

ttctccggcaagtaccaact

93

72

  

Reverse

cttccccttctggatgagc

  

I-FABP2

NM_000134

Forward

acaacctagcagacggaactg

78

1

  

Reverse

tccgtttgaattttccaataagtt

  

H-FABP3

NM_004102

Forward

ctgggcacctggaagcta

77

56

  

Reverse

ctggtagcaaaacccacacc

  

A-FABP4

NM_001442

Forward

cctttaaaaatactgagatttccttca

105

72

  

Reverse

ggacacccccatctaaggtt

  

E-FABP5

NM_001444

Forward

gcagacccctctctgcac

138

56

  

Reverse

tcgcaaagctattcccactc

  

IL-FABP6

NM_001445

Forward

ctcagagatcgtgggtgaca

68

22

  

Reverse

tcacgcgctcataggtca

  

B-FABP7

NM_001446

Forward

ctcagcacattcaagaacacg

69

33

  

Reverse

ccatccaggctaacaacagac

  

TBP

NM_003194

Forward

ttcggagagttctgggattgta

227

 
  

Reverse

tggactgttcttcactcttggc

  
   

Probe:

F-ccgtggttcgtggctctcttatcctcaT-P

  

PPIA

NM_021130

Forward

Hs_PPIA_I_SG

121

 
  

Reverse

QuantiTect Primer Assay

  
   

Qiagen

(Cat. No. QT00052311)

  
  1. * UPL probes from Universal ProbeLibrary; Roche