Figure 3

c-Myb activity is estrogen stimulated. (A) Comparison of ChIP-on-chip results obtained with MCF-7 cells that were deprived of estrogen for 48 hr followed by 24 hr of estrogen stimulation (Stimulated) or grown to high density (Confluent). The Venn diagram summarizes the statistically significant (P = 1×10^-5) binding sites identified in the two growth conditions. The complete list of binding sites is provided in Additional file 2, Additional file 3, Additional file 4, Additional file 5 and Additional file 6. (B) ChIP was performed on MCF-7 cells deprived of estrogen for 48 hr (gray bars) or deprived and then stimulated with 10 nM 17-beta-estradiol for 24 hr (black bars). Chromatin complexes were immunoprecipitated with anti-c-Myb 1.1 antibodies. Enrichment for the CXCR4, JUN, EPB41, CCNB1, and KLF4 promoters were measured by QPCR. Error Bars represent standard deviation of triplicate PCR reactions. (C) ChIP was performed as in (B) using MCF-7 cells expressing FLAG-tagged c-Myb and chromatin complexes were immunoprecipitated with anti-FLAG antibodies. Enrichment for the CXCR4, JUN, EPB41, CCNB1, and KLF4 promoters were measured by QPCR. Error Bars represent standard deviation of triplicate PCR reactions.