Figure 2
From: Identification and Regulation of c-Myb Target Genes in MCF-7 Cells
ChIP on chip identifies c-Myb target genes. (A) ChIP-on-chip was performed with anti-Myb antibodies 1493 and 1.1 using chromatin from MCF-7 cells that were deprived of estrogen for 48 hr followed by 24 hr of estrogen stimulation or grown to high density. The Venn diagram summarizes the statistically significant (P = 1×10-5) binding sites identified by each antibody in at least one of the two growth conditions. The complete list of binding sites is provided in Additional file 2, Additional file 3, Additional file 4, Additional file 5 and Additional file 6. (B) ChIP with anti-c-Myb antibodies. MCF-7 cells were subjected to chromatin immunoprecipitation with anti-c-Myb antibodies. Enrichment for the CXCR4, JUN, EPB41, CCNB1, and KLF4 promoters was assessed by QPCR. Fold enrichment was calculated relative to a control gene (GAPDH) and a control antibody (mouse non-specific IgG). Error bars show standard deviation of triplicate PCR reactions. (C) Conventional ChIP with anti-FLAG antibodies was performed on MCF-7 cells transduced with FLAG epitope-tagged c-Myb, using anti-FLAG antibodies. Fold enrichment was calculated as described above.