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Figure 4 | BMC Cancer

Figure 4

From: Loss of runt-related transcription factor 3 expression leads hepatocellular carcinoma cells to escape apoptosis

Figure 4

Effect of RUNX3 on apoptosis-related signaling molecules. (A) Immunoblot analysis Equal amounts of cell lysates were size-fractionated with 10% SDS-PAGE and immunoblotted with antibodies against Bax, Bcl-2, Bim, cleaved caspase-9, cleaved caspase-3, and actin. Shown here are representative blots from at least three independent experiments. (B) Apoptosis determined by DAPI staining Cells were cultured in media with or without 10% FBS, caspase inhibitor, and/or TGF-α for 48 h. Cells were stained with DAPI, and the percentage of apoptotic cells was determined under a fluorescent microscope (CAT; white bars, RUNX3; black bars). Data represent the mean ± S.E. of more than five independent experiments, each with triplicates. n.s., not significant; P > 0.05; **, P < 0.01 (vs. data with no serum); Student's t-test.

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