Figure 7
From: Differences in integrin expression and signaling within human breast cancer cells
Adhesion induced signaling of Hek-293 and breast cancer cells. Cells were either left in suspension or placed into wells coated with BSA, collagen (Coll), Fg and FN. After a one hour incubation at 37°C, plates were washed to remove non-adhered cells. Total cellular protein was then extracted from adhered and suspension cells and subjected to immunoblotting with antibodies against pFAK, pMEK and pERK as described in Figure 3. Blots for suspension cells are displayed in panel A. Blots for suspension and adhered cells are displayed in panel B. FAK, MEK, ERK, c-Src and pSrc levels were also assessed and remained unchanged (data not shown). (C) Samples were also probed using antibodies against Bcl2 and activated pErbb2. One of three representative experiments is shown.