Figure 2

Integrin expression by breast cancer and Hek-293 cells. A) Flow cytometric analysis of untreated cultured cells. Cells adhered to FN-coated dishes were harvested and incubated with the mouse anti-human β₁, β₃, β₅, α_v ,α_vβ₃ ,α_vβ₅ and α_vβ₆ antibodies, followed by incubation with fluorescent-labeled secondary antibody and then analyzed by flow cytometry (black lines). Isotype-matched irrelevant antibodies were used as controls (grey lines). B) Flow cytometric analysis of integrin expression by PMA stimulated cells. Adhered cells were incubated overnight in media containing 1% fetal calf serum and then stimulated with 150 nM PMA for two hours. The cells were then harvested and integrin expression levels assessed as described in panel A. In control plates (mock treatment), an equal quantity of DMSO was added instead of PMA. One of three representative experiments is shown.