Dose and polymorphic genes xrcc1, xrcc3, gst play a role in the risk of developing erythema in breast cancer patients following single shot partial breast irradiation after conservative surgery

Table 1 Polymorphic genes with reference sequence [rs], PCR and sequence primers, products size and annealing conditions

Gene	NCBI dbSNP ID	Primers	PCR product size (bp)	PCR Annealing temp.
XRCC1 Arg194Trp	[rs1799782]	PCR Forward: 5'-GCCGGGGGCTCTCTTCTT-3' PCR Reverse biot: 5'-CTCACTCAGGACCCACGTTGT-3' Pyrosequence: 5'-GGGGCTCTCTTCTTCA-3'	75bp	62°C
GSTA1	[rs3957356]	PCR Forward: 5'-GGCTCGACAACTGAATTCCA-3' PCR Reverse biot: 5'-TGGCTTTTCCCTAACTTGACTCT-3' Pyrosequence: 5'-GACTCTTCTTTCAGTGGG-3'	101 bp	56°C

Abbreviations: NCBI = National Center for Biotechnology Information, ID = identification.

ISSN: 1471-2407