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Table 1 Polymorphic genes with reference sequence [rs], PCR and sequence primers, products size and annealing conditions

From: Dose and polymorphic genes xrcc1, xrcc3, gst play a role in the risk of developing erythema in breast cancer patients following single shot partial breast irradiation after conservative surgery

Gene NCBI
dbSNP ID
Primers PCR
product
size (bp)
PCR
Annealing
temp.
XRCC1 Arg194Trp [rs1799782] PCR Forward:
5'-GCCGGGGGCTCTCTTCTT-3'
PCR Reverse biot:
5'-CTCACTCAGGACCCACGTTGT-3'
Pyrosequence:
5'-GGGGCTCTCTTCTTCA-3'
75bp 62°C
GSTA1 [rs3957356] PCR Forward:
5'-GGCTCGACAACTGAATTCCA-3'
PCR Reverse biot:
5'-TGGCTTTTCCCTAACTTGACTCT-3'
Pyrosequence:
5'-GACTCTTCTTTCAGTGGG-3'
101 bp 56°C
  1. Abbreviations: NCBI = National Center for Biotechnology Information, ID = identification.