Figure 4

DR3 activation triggers NFκB activation through PI3K. HT29 cells were pre-treated for 2 hours with PI3K inhibitor LY294002 (20 μM) or DMSO as vehicle (0.1%) and treated or not with rhE-selectin/Fc (5 ng/ml for 15 min). Nuclear proteins were extracted and processed for western blotting using specific antibodies for total p65 NFκB or for TATA binding protein (TBP) used as nuclear protein control.