Figure 4

Effects of pitavastatin on the serum levels of TNF-α and the expression levels of TNF-α and IL-6 mRNAs in the liver of DEN-treated db/db mice. (A) The serum concentration of TNF-α was measured by enzyme immunoassay. Values represent the means ± SD. * P < 0.05 vs. the untreated group. (B) The total RNAs were isolated from the livers of experimental mice and the expression levels of TNF-α and IL-6 mRNAs were examined by quantitative real-time RT-PCR using specific primers. The expression levels of each mRNA were normalized to the level of GAPDH mRNA. Values represent the means ± SD. * P < 0.05 vs. the untreated group.