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Table 2 Oligonucleotide primer sequences used for the amplification of different regions of HPV and internal control.

From: Breast cancer and human papillomavirus infection: No evidence of HPV etiology of breast cancer in Indian women

Primers Primer Sequences Location Annealing
temperature
(ºC)
Amplimer size
(bp)
Reference
MY09 5'-GCM CAG GGW CAT AAY AAT GG-3' L1 55 450 [49]
MY11 5'-CGT CCM ARR GGA WAC TGA TC-3' where M = A + C, W = A + T, Y = C + T, R = A + G).     
GP5+ 5'-TTT GTT ACT GTG GTA GAT ACT AC-3' L1 40 145 [51]
GP6+ 5'-CTT ATA CTA AAT GTC AAA TAA AAA-3'     
HPV16 E6 (F) 5'-GAA ACC GGT TAG TAT AAA AGC AGA C-3' 53-78 55 503 [49]
HPV16 E6 (R) 5'-AGC TGG GTT TCT CTA CGT GTT CT-3' 557-533    
HPV16 E7 (F) 5'-CAA TAA TAT AAG GGG TCG GTG GA-3' 480-500 55 468  
HPV16 E7 (R) 5'-TTT TTC CAC TAC AGC CTC TAC AT-3' 945-923    [49]
β-globin (F) 5'-GAA GAG CCA AGG ACA GGT AC-3' 4-23 55 268 [49]
β-globin (R) 5'-CAA CTT CAT CCA CGT TAC ACC-3' 273-256    
p53 Exon 5 (F) 5'-TAC TCC CCT GCC CTC AAC AA-3' 316-355 55 184 [48]
p53 Exon 5 (R) 5'-CAT CGC TAT CTG AGC AGC GC-3' 499-480