JSI-124 induces phosphorylation of c-Jun in B-leukemia cells. A, and B. I-83, BJAB, NALM-6 cells were treated with 1 μM JSI-124 for a 6 and 24 hour time course. Cell lysates were used for immunoblotting for the presence of p-JNK/JNK, p-p38/p38, p-p42/44 or p42/44 and p-c-Jun/c-Jun. Experiments were done at least in triplicate for all cells and for primary CLL cells. Representative original data are shown in Fig.1. C. All three cell lines were treated with 1 μM JSI-124 for 6 hours. D. Cells were treated with various doses of JSI-124 for 6 hours. Phospho-and total c-Jun levels were assessed by immunoblotting. E. BJAB, I-83, and NALM-6 cells were treated with 0.2 μM concentration of JSI-124 for 24 hour. Apoptosis was measured using flow cytometric analysis for accumulation of sub-G1 phase (upper panel) or Annexin V/7AAD staining. Data represents 3 independent experiments. Standard error was determined on the basis of three independent experiments.