Figure 2

Detection of different mutations within the same amplicon ( BRCA1 11I region). The left panel shows the difference curves and the right panel shows the corresponding melting peak curves. All mutations produce obvious biphasic melting which is caused by the earlier melting of the heteroduplexes. The normalised and temperature-shifted difference plots in the left panel allow easy detection of the c.2863delTCATC (navy), c.2800C>T (red) and c.2885delA (green) relative to the wildtype HRM profile (grey). The melting peak curves in the right panel show that the c.2885delA is the most subtle mutation in that there is a minimal early melting heteroduplex component compared to the c.2863delTCATC and c.2800C>T mutations.