Figure 3

Effect of FOXO1 inhibition on HIF-1α expression under hypoxic conditions in vitro. SNU-638 gastric cancer cells were infected with a lentivirus containing a construct, encoding either FOXO1 shRNA (denoted as shFOXO1) or non-targeting shRNA (denoted as LVcontrol). Expression levels of HIF-1α and β-actin proteins were determined by Western blotting (WB) and the mRNA expressions of HIF-1α and β-actin were determined by SQ RT-PCR (RT-PCR) after cells were exposed to either normoxia (N) or hypoxia (H) for 8 h.